Effect of copper, nickel and lead on callus growth dynamics of Solanum lycopersicum

Solanum lycopersicum cv. (Punjab Kesar Cherry) (Tomato) belonging to family Solanaceae has been priced for the presence of lycopene, low sugar content and curative properties. However, productivity tomatoes observed be due both biotic as well abiotic stress. Considering toxicity copper (Cu), lead (Pb) nickel (Ni) environmental contamination economic importance S. lycopersicum, present study was undertaken investigate effects these heavy metals on various growth parameters callus cultures plantlet regeneration. Callus induced through nodal segments inoculated Murashige Skoog medium containing different concentrations (0 µM, 50 100 200 300 400 µM 500 µM) copper, nickel. Decrease in 91.66-16.66 %, 76.38-11.11 81.94-13.88 % percent survival, 90.90-25 72.72-37.50 79.66-20 multiplication, 79.54-0 82.75-33 63.8-50 root regeneration 73.33-33 72.50-33 79.6- 20 shoot generation with increasing Cu, Pb Ni, respectively. average fresh dry weight all metals. The order > Ni Cu at highest used treating lycopersicum. revealed that three stress studied plant need attention developing methods mitigate consequences metal crop plants.